Re-Testing: To test a single guide, please provide a guide sequence between 18-23 nucleotides long. Example: GAGGCGUCAUCGAUGACCGA
The default sgRNA target site is 20 nucleotides, but can be longer to incorporate promoter requirements for transcription. If transcribed from a U6 promoter, the first nucleotide needs to be a G, but this G does not need to be present in the targeted genome sequence. If transcribed in vitro from a T7 promoter, the first three nucleotides need to be G for efficient transcription. If the sgRNA is longer, additional nucleotides need to be added for folding.
Privacy | Legal | Freedom of Information | Cookies | Accessibility