PlatinumCRISPr

Identify Cas9 sgRNAs in Genomic Sequence

Paste sequence below
      or
Select sequence by gene coordinates

Paste a sequence below or retrieve one from Ensembl using gene coordinates. PlatinumCRISPr will identify candidate Cas9 sgRNAs and evaluate folding and efficiency predictions.

Fetch gene sequence from Ensembl

Organism: Please select Human M. musculus D. rerio D. melanogaster A. thaliana C. elegans Other Vertebrate Other Invertebrate Other Plant    Gene symbol:    Fetch gene

Scan for sgRNAs

Organism (for scanning of off-targets) Please select Human M. musculus D. rerio D. melanogaster A. thaliana C. elegans Other Vertebrate Other Invertebrate Other Plant

Target sequence (DNA; max 2000 bp)

Search ID (optional)

The selected gRNAs are 20 nucleotides and incorporate a G in the first nucleotide position, either present in the sequence or added artificially. (Requirement for U6 or U7 promotors). If requirements are different, please select the guide and re-test below. Re-testing is essential because single-nucleotide changes can alter RNA folding.

Load Example Sequence

Re-test a single sgRNA

Enter an sgRNA between 18–23 nucleotides for RNA structure evaluation.

The default sgRNA is 20 nucleotides, but can be longer to incorporate promoter requirements for transcription. If transcribed from a U6 promoter, the first nucleotide needs to be a G, but this G does not need to be present in the targeted genome sequence. If transcribed in vitro from a T7 promoter, the first three nucleotides need to be G for efficient transcription. Since adding even a single nucleotide can alter RNA folding, the whole sgRNA sequence needs to be tested.

Guide sequence

Guide ID (optional)

Re-test guide Split crRNA and tracrRNA Load Example sgRNA